Proteins were then transferred into nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), blocked in 5% BSA for 1h at room heat. exosomes exerted its effect within a shorter time compared to that induced by its endogenous manifestation. The difference of ITGA2 protein manifestation in localized tumors and those with lymph node metastatic cells was indistinguishable. However, … Continue reading Proteins were then transferred into nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), blocked in 5% BSA for 1h at room heat